Strep. A test
MCL Procedure for Waived
Specimen Collection Instructions
- The kit is stored at room
- Using only the sterile rayon
swab that comes with this test, rub the swab on the back of the throat, on
the tonsils and in other area of redness, inflammation or pus. Be careful
not to touch the tongue, teeth, gums or cheek. Do not use any other
swabs expect the ones that come with this kit. Process the swab as
soon as possible
- Add three drops of reagent 1
and three drops of reagent 2 to the test tube.(the solution should be
Caution: Reagent 2 contains and acid. If solution
contacts skin or eyes, flush with large quantity of water.
- Immediately put the swab into
the tube and vigorously mix by rotating against the side of the tube for a
minimum of ten times. Through extraction will ensure best results
- Let the swab stand for one
minute in the solution then express as much liquid as possible from the
swab as it is removed. Squeeze the sides of the tube during extraction to
express the liquid from the swab. Once removed, discard the swab.
- Remove a test stick from its
- Place the absorbent end of
the test strip into the sample solution.
- Read results at five minutes.
- Record the results: Write
your name, the date, time and findings on the patient record.
Confirm all negative findings with a throat culture.
- Record the results on the
Strept A Patient and Control Form.
Express liquid after one minute.
Read result after five minutes.
Internal Control must be visible (red line) before a
result is valid. Two lines-- blue and red indicate a positive result. Red
line alone means a negative result.
The internal quality control is in the form of a red control line. The control
line must appear in the proper location before the test can be accepted. A
positive finding in the control line means that: there is functional activity
of the detection component of the test
- External quality control in
the form of positive and negative materials must be performed when a new
kit is opened or when a new operator performs the test for the first time.
Failures in quality control must be corrected by re-testing the controls
before any patients are tested. A
second failure in quality must be corrected by acquiring a new kit.
- Failures of the quality
control must be documented by nursing service report and forwarded to the
laboratory for full investigation.